PREPARATION OF ISOLATED RAT LIVER MITOCHONDRIA FOR ELECTRON MICROSCOPY
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چکیده
منابع مشابه
Preparation of Isolated Rat Liver Mitochondria for Electron Microscopy
A comparative study of the fixation of isolated rat liver mitochondria was undertaken. If the criterion is adopted that after processing, the mitochondria should resemble as closely as possible rat liver mitochondria in situ, the procedure found to produce such preservation was that of fixation in suspension in veronal-buffered 2% potassium permanganate. Fixation in osmium tetroxide produced va...
متن کاملThe action of digitonin on rat liver mitochondria. Electron microscopy.
1. Rat liver mitochondria were examined in the electron microscope by using negative staining in the presence of 0.3m-sucrose. The intact outer membrane does not appear to be freely permeable to the stain. Where the stain penetrated through a tear it was seen that the inner membrane had randomly oriented grooves, many of which contained round structures varying between 200 and 900å in diameter....
متن کاملSTUDY OF MITOCHONDRIA IN RAT LIVER Quantitative Electron Microscopy
The electron microscope has been used to determine the weight distribution of isolated subcellular particles from normal rat liver. The following results are reported: (1) There exist at least two well defined weight populations of subcellular particles; their respective median weights are 1.3 X l0 -j4 and l l X l0 u gm. The lighter fraction is considered to consist of lysosomes, the heavier of...
متن کاملSTUDY OF MITOCHONDRIA IN RAT LIVER Quantitative Electron Microscopy
The electron microscope has been used to determine the weight distribution of isolated subcellular particles from normal rat liver. The following results are reported: (1) There exist at least two well defined weight populations of subcellular particles; their respective median weights are 1.3 X l0 -j4 and l l X l0 u gm. The lighter fraction is considered to consist of lysosomes, the heavier of...
متن کاملSTUDY OF MITOCHONDRIA IN RAT LIVER Quantitative Electron Microscopy
The electron microscope has been used to determine the weight distribution of isolated subcellular particles from normal rat liver. The following results are reported: (1) There exist at least two well defined weight populations of subcellular particles; their respective median weights are 1.3 X l0 -j4 and l l X l0 u gm. The lighter fraction is considered to consist of lysosomes, the heavier of...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 1970
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.44.2.278